Current Research in Protein Chemistry

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Current Research in Protein Techniques, Structure, and Function explores the methods for determining protein structure and function. It covers a range of topics, including protein folding, stability, chimeric protein catalysis, amino acid and peptide analysis, mass spectrometry applications, and protein sequencing. The book is organized into six sections with 55 chapters. The opening chapter presents a novel microwave irradiation method for protein hydrolysis using Teflon-Pyrex tubes, followed by a discussion on high-performance capillary electrophoresis for amino acid analysis. Subsequent sections delve into mass spectrometric techniques, protein sequencing, and capillary electrophoresis, alongside topics like protein stability, enzyme modifications, and structure prediction. Key subjects include the crystal structure of human interleukin-1alpha, acid-denatured protein states, solubility of recombinant proteins in Escherichia coli, and chimeric protein catalysis. Additionally, the book introduces peptide mapping, internal sequencing from acrylamide gels, new covalent sequence analysis techniques, alkaline hemoglobin denaturation, and disulfide bond stability measurements in protein folding intermediates. This resource is invaluable for students and researchers in protein chemistry.