Protein Methods

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This revised and expanded Second Edition remains a comprehensive source for proven protein techniques, now divided into two parts. It begins with essential chapters from the first edition, updated to reflect significant methodological advancements. Notably, the chapter on protein isolation includes a new section on isolating proteins from inclusion bodies. The second section introduces four new chapters focused on protein purification and crystallization. Chapters cover a range of topics, including preparation for protein isolation, extraction and solubilization, concentration determination, and gel electrophoresis under various conditions. Each chapter includes detailed information on required reagents and equipment, "view-at-a-glance" tabs for easy navigation, and illustrations for gel applications and other methods. Additional features include a complete supplier list, appendices with basic chemical parameters and molecular data, key references, descriptive tables, and safety notes. Reviews of the first edition praised its clarity and practicality, highlighting its usefulness for both beginners and experienced scientists. The book is designed to provide readers with the necessary information to perform techniques effectively, minimizing the need for additional reference sources.